Abstract Detail

Genomics / Proteomics

MacArthur , Kenton  [1], Murray, Kevin [2], Neupane, Achal [2], Benson, Benjamin [2], Piya, Sarbottam [2], Nepal, Madhav [3].

Evolution of Mitogen Activated Protein Kinase Kinase (MAPKK) Gene Family in Plants.

Plants have developed sophisticated mechanism where their cells perceive, propagate and translate intracellular and extracellular stimuli through signal transductions. Such signal transductions involve a set of genes called Mitogen Activated Protein Kinases (MAPKs) genes, which occur in three functionally linked families MAPK, MAPKK and MAPKKK. In our recent in-slico analysis, our lab has identified 38 MAPKs, 11 MAPKKs and 115 MAPKKKs in soybean genome. Increasing availability of complete genome sequences from multiple plant species across the taxonomic group allows us to conduct comparative analyses and infer evolutionary relationships. The main objectives were to study phylogenetic relationships among the gene members across plant species and make evolutionary inferences from the analyses of - Ka/Ks ratios, GC content and RNAseq data. To accomplish these objectives, genomic and protein sequences of MAPKK genes were searched thoroughly using Arabidopsis MAPK gene sequences as reference. MAPKK orthologs in eight plant species including four legume and four non-legume species are included for the combined phylogenetic analysis. We are analyzing Ka/Ks ratios, GC content and RNAseq data available in public databases. We will present our results about genealogical relationships, rate of evolution and functional divergence of MAPKK genes in plants with a focus in legumes.

Broader Impacts:

1 - South Dakota State University, Biology and Microbiology, Nepal Lab, SNP213, Brookings, South Dakota, 57007, United States
2 - South Dakota State University, Nepal Lab, SNP213, Brookings, South Dakota, 57007, United States
3 - South Dakota State University, Biology And Microbiology, Northern Plain Biostress Laboratory, Brookings, SD, 57007, USA

Keywords:
MAPK genes
gene family
gene duplication
Evolutionary divergence
paralogous genes.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PGP009
Abstract ID:962
Candidate for Awards:Genetics Section Poster Award