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Abstract Detail

Systematics Section/ASPT

Kates, Heather [1], Soltis, Douglas [2], Soltis, Pamela [3].

Reconstructing the domestication histories of pumpkins and squashes (Cucurbita) using whole plastome sequences and single copy nuclear genes.

Investigating the genetic origins of domesticated plants can reveal details about the domestication process and the genetic properties of the domesticates themselves. A phylogenetic study of pumpkins and squashes (Cucurbita) using a set of markers derived from introns of single-copy nuclear genes provides new insights into the relationships between wild and cultivated species in the genus and their domestication histories. The keys to this study that allow me to address previously unresolved questions are: 1) increased taxon and population sampling compared with previous studies, and 2) the use of novel genetic markers that have great utility at the population and species levels. I am analyzing a set of Cucurbita accessions that cover the geographic and taxonomic range of the genus using whole plastome sequencing and multiple singe-copy nuclear markers developed with a bioinformatics pipeline that utilizes genomic and transcriptomic resources for Cucurbitaceae. The results from these analyses are being used to resolve relationships within Cucurbita and provide new insights into its multiple domestications, while demonstrating the utility of a bioinformatics approach to optimizing phylogenetic marker development.

Broader Impacts:

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1 - University of Florida, Biology, Florida Museum of Natural History, Dickinson Hall, PO Box 117800, Gainesville, Florida, 32611, United States
2 - University of Florida, Biology and Florida Museum of Natural History, Gainesville, FL, 32605, USA
3 - University of Florida, Florida Museum of Natural History, PO BOX 117800, Gainesville, FL, 32611-7800, USA

low copy nuclear markers

Presentation Type: Oral Paper:Papers for Sections
Session: 5
Location: Rosedown/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 11:45 AM
Number: 5013
Abstract ID:863
Candidate for Awards:None

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