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Abstract Detail



Symbioses: Plant, Animal, and Microbe Interactions

Hernandez-Quezada, David [1], Yigit, Erbay [2], Bailey, C. Donovan [1].

An investigation of vertically transmitted endophytes in Leucaena leucocephala (Leguminosae: Mimosoideae) using a novel approach for prokaryotic DNA enrichment.

Recent interest and discoveries on bacterial endophytes suggest that the diversity of endophytes is greater than previously thought. Numerous endophytes have been discovered that provide a diverse array of potential benefits to the host plant, including symbioses that aid in nutrient acquisition, that regulate and enhance plant growth patterns, or that provide protective agents or properties. Here we investigate the vertically transmitted Bacterial microbiome of Leucaena leucocephala using genomic DNA extractions from week old seedlings derived from surface sterilized seeds raised under sterile conditions. First, we employ a bacterial specific 16S PCR-based screen and a phylogenetic approach that includes samples of all major lineages of Eubacteria. Second, we implement a novel approach, being developed by New England Biolabs, to enrich for prokaryotic DNA prior to Illumina sequencing and MetaPhAn analysis to more fully characterize the microbiome of L. leucocephala. Preliminary results from the first element have recovered DNA sequences from lineages related to Herbaspirillum and Stenotrophomonas, groups that include potential nitrogen fixing and iron reducing bacteria, but that do not appear to have been previously isolated from legume tissues. Preliminary results from the second approach suggest that a diverse array of endophytes are transmitted through Leucaena seeds, including representatives of as many as 10 Bacterial phyla.

Broader Impacts:


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1 - New Mexico State University, Biology, PO Box 30001 Msc 3AF, Las Cruces, NM, 88003, USA
2 - New England Biolabs, Research and Development, 240 County Road, Ipswich, MA, 01938-2723, USA

Keywords:
Leucaena
endophyte
microbiome
Illumina
Eubacteria
bacteria.

Presentation Type: Oral Paper:Papers for Topics
Session: 8
Location: Prince of Wales/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 9:15 AM
Number: 8005
Abstract ID:828
Candidate for Awards:None


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