| Abstract Detail
Phylogenomics Benson, Benjamin [1], Nepal, Madhav [1]. Diversity and Evolutionary Divergence of Resistant Genes (R Genes) in Legumes. Proteins coded by Resistance Genes (R genes) play important role in plant’s defense against diverse biotic stresses. Out of the five families of R genes in plants, Nucleotide Binding Site Lucien Rich Region (NBS-LRR) is the largest family. The NBS-LRR family is divided into two groups: TIR-NBS-LRRs (TNLs) that contain Toll/Interleukin-1 Receptor (TIR) and CC-NBS-LRRs (CNLs) that do not contain the TIR domain. Recent availability of genome sequences of increasing number of plant species allows us to perform the phylogenomic analyses of the NBS-LRR genes, and study their diversity and evolutionary divergence across species. Using approaches in bioinformatics and molecular phylogenetics, we aimed to identify CNL group of NBS-LRR genes and elucidate their evolutionary divergence across legume species. Using Arabidopsis thaliana R-gene amino acid sequences as reference sequences, we searched for their orthologs in five plant species Glycine max, Medicago truncatula, Phaseolus vulgaris, Oryza sativa and Populus trichocarpa. From the resulting 30,000 sequences, duplicates were removed leaving 1209 sequences that included 891 CNL gene sequences. We identified a total of 187 CNL genes in Glycine max, 201 in Medicago truncatula and 126 in Phaseolus vulgaris, 49 in Arabidopsis thaliana, 129 in Oryza sativa and 199 in Populas trichocarpa. Phylogenetic analyses of these gene sequences revealed that the orthlogs of the R-genes belonging to four CNL subgroups (A-D) of Arabidopsis were present in all species studied. Analysis of paralogous relationships among multiple CNL genes is under way. Results from the analysis of structural variation, functional coherence (expression profiles) and evolutionary divergence of the CNL genes in six plant species will be presented. Broader Impacts:
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1 - South Dakota State University, Biology And Microbiology, Northern Plain Biostress Laboratory, Brookings, SD, 57007, USA
Keywords:
CNL
NBS-LRR
legumes
functional divergence of gene duplicates
Diversity.
Presentation Type: Poster:Posters for Topics
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PPL003
Abstract ID:784
Candidate for Awards:None |
