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Abstract Detail

Genetics Section

Wagner, Jennifer [1], Noyes, Richard [2].

Evaluation of genome size variation in Erigeron (Asteraceae) via flow cytometry.

Genome size estimates along with chromosome numbers establish a starting point for studies of genome evolution, structure, and sequence. Erigeron (Asteraceae) is a large genus of annual and perennial herbs that occupies diverse ecological niches. A few species of Erigeron have been studied in depth in the context reproductive and adaptive evolution and have been tapped for genome sequencing. However, among the 400 species in the genus, only 2 genome size estimates have been reported (0.5%). In this study we investigate genome size for a ploidal series in Erigeron strigosus plus 12 other species representing a diversity of sections within the genus. Leaf nuclei were isolated using a standard buffer and analyzed using a Beckman Coulter Cell Lab Quanta flow cytometer. Pisum sativum ("Maple Minerva") leaf nuclei served as a size standard. We report the first nuclear genome size estimates for these species, report variation within the genus, and compare genome size estimates for Erigeron with those of other Asteraceae.

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1 - University of Central Arkansas, Department of Biology, 180 Lewis Science Center, Conway, AR, 72034, USA
2 - University of Central Arkansas, Department of Biology, Lewis Science Center 180, Conway, AR, 72035, USA

flow cytometry

Presentation Type: Oral Paper:Papers for Sections
Session: 21
Location: Marlborough B/Riverside Hilton
Date: Tuesday, July 30th, 2013
Time: 9:30 AM
Number: 21004
Abstract ID:782
Candidate for Awards:None

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