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Abstract Detail


Carlsen, Monica [1], McKain, Michael [2], Kellogg, Elizabeth [3].

Using Next-Gen sequencing to improve phylogenetic resolution in a rapid radiation of species, Anthurium (Araceae), preliminary data.

Anthurium is an extremely diverse and strictly neotropical genus of Araceae that includes ca. 1,000 species. The most recent molecular phylogeny of the genus includes 102 Anthurium species and eight outgroups, and was able to distinguished 18 highly supported clades within the genus. That phylogeny was generated using standard Sanger sequencing of chloroplast (trnG intron, trnH-psbA and trnC-ycf6 intergenic spacers) and nuclear (CHS first intron) DNA regions. This dataset also revealed a molecular signature of very low DNA sequence differentiation among Anthurium species, consistent with a pattern of rapid and recent species radiation, originating approximately 5-8 Mya following the uplift of the Andes. Not surprisingly, relationships among the 18 major clades were not supported, and the backbone of the phylogeny was still unresolved, therefore precluding further assessments of character evolution within the genus. In this preliminary study, we used Next-Gen sequencing to generate entire chloroplast genomes for 22 Anthurium species representing most major clades in the previous phylogeny, in order to investigate the amount of data necessary to improve the phylogenetic resolution within the genus. Plastomes for outgroups were assembled from publically available data, and represent other aroid genera, Acorus and Alismataceae. As expected, preliminary data suggest that the use of full chloroplast genome alignments greatly increases the number of informative sites and phylogenetic support in deeper nodes of the phylogeny. Moreover, some areas of the plastome were identified as providing a higher number of informative characters, and thus could be targeted for subsequent studies. Using a supertree approach, both phylogenetic estimates (i.e. Sanger-based and Next-Gen based) were combined to produce a better resolved picture of the evolutionary history of Anthurium, and to highlight areas still in need to improvement.

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1 - Missouri Botanical Garden, PO Box 299, St. Louis, MO, 63166, USA
2 - University of Missouri-St. Louis, Plant Biology, R223 Research Builiding, One University Blvd., St. Louis, MO, 63121, USA
3 - University of Missouri - St. Louis, Department of Biology, One University Boulevard, St. Louis, MO, 63121-4499, USA, 314/516-6217

rapid radiation
chloroplast genome.

Presentation Type: Oral Paper:Papers for Topics
Session: 36
Location: Magnolia/Riverside Hilton
Date: Tuesday, July 30th, 2013
Time: 4:45 PM
Number: 36013
Abstract ID:763
Candidate for Awards:None

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