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Abstract Detail



Systematics Section/ASPT

Mabry, Makenzie [1], Simpson, Michael [2].

Phylogenetic systematics of Cryptantha s.s. (Boraginaceae): Use of next generation sequencing to provide enhanced infrageneric resolution.

Cryptantha s.l. (Boragainceae) has recently been split into five genera (Cryptantha s.s., Eremocarya, Greeneocharis, Johnstonella, and Oreocarya), as based on a molecular phylogenetic study using ITS and trnLF sequence data. These genera are mostly well-defined on morphological criteria, including aspects of perenniality, floral bract presence, nutlet heteromorphism, and nutlet sculpturing morphology. The large Cryptantha s.s. group was resolved into two clades, which are sister taxa in one analysis but well separated in another. Relationships within the Cryptantha s.s. clades were generally not well-supported. Here we present the use of next generation sequencing (NGS) methods to better resolve relationships within and between these clades. For this study, 100-bp single-end Illumina reads will be generated for approximately 25 species of Cryptantha s.s. (in both major clades) and outgroups. The “genome skimming“ method of reference-guided assembly will be used to recover sequences of the plastome and nrDNA cistron. De novo assembly of read data will be used to find orthologous markers shared among sample taxa. Maximum Likelihood and Bayesian methods will be used to generate trees. This study will test if this aspect of next generation sequencing will provide better resolution to infer both relationships at lower levels and the interrelationships of the Cryptantha s.s. 1 and s.s. 2 clades. Better resolution of these clades will allow testing biogeographic history, character evolution, and divergence times in the complex.

Broader Impacts:


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1 - San Diego State University, Evolutionary Biology, 5500 Campanile Dr., San Diego, CA, 92182, USA
2 - San Diego State University, Department of Biology, Department Of Biology, San Diego State University, San Diego, CA, 92182-4416, USA

Keywords:
next generation sequencing
Cryptantha.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PSY043
Abstract ID:741
Candidate for Awards:None


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