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Abstract Detail



Systematics Section/ASPT

Hoose, Dalton [1], Willyard, Ann [2].

Pipelines to Assemble Next Gen Sequences for the Pine Tree of Life.

Resolving a species-level Pinus phylogeny has proven to be a difficult endeavor due to incomplete lineage sorting. As Next-Gen sequencing has become more refined, it has become an increasingly beneficial tool to aid in analysis of non-model species by facilitating a large number of independent gene trees that can be used to create a species tree. We developed a pipeline to execute existing software modules and new programs to assemble short reads via reference-guided assembly, select genes that assembled with the highest scores, create multi-sequence alignments, annotate protein-coding regions, and build gene trees to show relationships between individuals. The development of this pipeline makes it possible to streamline the processing of these enormous amounts of data. Continued research can analyze more genes and strengthen support for evident links.

Broader Impacts:


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1 - Hendrix College, Biology Department, 1600 Washington Ave, Conway, AR, 72032, USA
2 - Hendrix College, Biology Dept, 1600 Washington Ave, Conway, AR, 72032, USA

Keywords:
next generation sequencing
species tree
Low-copy nuclear gene.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PSY017
Abstract ID:540
Candidate for Awards:None


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