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Abstract Detail



Systematics Section/ASPT

Mandala, Venkata Shiva [1], Stull, Gregory [2], Brockington, Samuel [3], Gitzendanner, Matthew [4], Douglas, Norman [5], Soltis, Douglas [6], Soltis, Pamela [7], Moore, Michael [8].

Clarifying the backbone relationships of Caryophyllales using complete plastomes.

Caryophyllales is one of the oldest angiosperm orders with 34 families and over 11,500 species, and constitutes around 6.3% of eudicot diversity. Caryophyllales possess enormous ecophysiological diversity, ranging from cacti and ice plants to carnivorous pitcher plants and sundews, and are found in all terrestrial ecosystems, although they are most common in arid and alkaline regions. The monophyly of Caryophyllales has been well supported in all previous studies, although many relationships along the backbone of Caryophyllales remain poorly supported. For example, relationships among the major lineages of the phytolaccoid clade (Sarcobataceae, Agdestidoideae, Rivinoideae, Phytolaccoideae, and Nyctaginaceae) are only weakly supported, as are the positions of several small families or lineages, including Stegnospermataceae and Macarthuria. To resolve these relationships, we obtained complete or nearly-complete plastomes for 38 species of Caryophyllales, including exemplars of nearly all families (24) in the order and all major subclades of the phytolaccoid clade. Complete plastome data were obtained using the Illumina platform and were sequenced either from total genomic DNA libraries or using a plastome-based targeted enrichment strategy incorporating an Agilent SureSelect bait array. For phylogenetic analysis, gene sequences for all 79 protein-coding genes plus the four rRNA genes were extracted from each new Caryophyllales genome and were then added to an existing 83-gene data set (Moore et al. 2010) including exemplar taxa from nearly all angiosperm orders. Aside from providing new phylogenetic insight into the relationships of Caryophyllales, we also examine plastid genome structure in three carnivorous members of Caryophyllales (Drosera, Drosophyllum, and Nepenthes), which represent the first plastid genomes to be sequenced for any carnivorous plants. We also report on the unexpectedly highly rearranged plastome of Pharnaceum (Molluginaceae).

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1 - Oberlin College, Department of Biology, 119 Woodland St. , Oberlin, OH, 44074, USA
2 - University of Florida, Florida Museum of Natural HIstory, Dickinson Hall, Museum Rd. And Newel Dr., Gainesville, N/A, 32611, USA
3 - Department of Plant Science, Downing Site, Cambridge, N/A, CB23AE, United Kingdom
4 - University of Florida, Florida Museum of Natural History, PO BOX 117800, GAINSVILLE, FL, 32611-7800, USA
5 - Oberlin College, Biology, 119 Woodland St., Oberlin, OH, 44074, USA
6 - University of Florida, Biology and Florida Museum of Natural History, Gainesville, FL, 32605, USA
7 - University of Florida, Florida Museum of Natural History, PO BOX 117800, Gainesville, FL, 32611-7800, USA
8 - Oberlin College, 119 Woodland St., Science Center K111, Oberlin, OH, 44074, USA

Keywords:
Caryophyllales
Phylogenetics
plastid sequences
next generation sequencing.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PSY040
Abstract ID:533
Candidate for Awards:None


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