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Abstract Detail



Systematics Section/ASPT

Wofford, Austin [1], Willyard, Ann [2].

Placing plastid microsatellites on a genomic framework .

Defining the genetic parameters of plant species and populations is an important element in estimating the need for conservation efforts. Plastid microsatellite genotypes are one useful tool for the study of plant population genetics because they are highly polymorphic. We explored 15 microsatellite loci that have been frequently used in species delimitations of Pinus (Pinaceae). Because these loci were identified before plastid genomes were available, their location on the plastid genome has not been established. We obtained nucleotide sequences for these fragments in one plant sample of Pinus ponderosa and aligned them to the recently published P. ponderosa plastid genome in order to test whether these simple repeat regions are located in highly variable regions of the genome. We also determined how well these primers are conserved across the entire genus by aligning the primers to the published plastid genomes of all other species of Pinus. We discuss the genetic characteristics of these 15 loci and their usefulness for phylogenetic questions across the pines.

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1 - Hendrix College, Biology Department, 1600 Washington Ave, Conway, AR, 72032, USA
2 - Hendrix College, Biology Dept, 1600 Washington Ave, Conway, AR, 72032, USA

Keywords:
microsatellites
plastid.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PSY014
Abstract ID:469
Candidate for Awards:None


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