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Abstract Detail



Systematics Section/ASPT

Meersman, Benjamin [1], Olmstead, Richard [2].

Primer Development for the Pentatricopeptide Repeat Gene Family for Use in the Large Plant Group Lamiales.

Phylogenetics is the study of evolutionary relationships and in order to infer these relationships in plants, various loci that are phylogenetically useful in nuclear as well as chloroplast DNAs should be utilized. Presently, the use of chloroplast loci is widespread in phylogenetic studies whereas the nuclear genome is still somewhat under-utilized. The pentatricopeptide repeat (PPR) gene family is a large group of protein coding nuclear genes that has been shown to be highly informative in the inference of evolutionary relationships among closely related species. There are multiple reasons that make these genes useful to these types of studies. The PPR gene family is very large which offers researchers multiple loci that are available for phylogenetic analyses. This is important because having multiple loci is imperative to answering important phylogenetic questions. A large portion of PPR genes are intronless making it possible to sequence and align data with little or no difficulty. They also have a high rate of evolution, and are single-copy in most plant genomes. 127 of these loci have been identified as phylogenetically useful but of these only 5 have been developed. This research looks to develop 10 more loci that will specifically target the Lamiales. To do this, 10 primer sets were tested using a sampling of species across the Lamiales. The primers were used to amplify these loci in order to ascertain their usefulness for a wide range of species and to obtain sequence data for each species. The sequences obtained were aligned and the alignments were used to design new primers. These new primer sets will allow for new data to be more easily collected that has been either difficult to obtain or previously unavailable and will increase the number of nuclear loci available for phylogenetic studies.

Broader Impacts:


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1 - University of Washington, Biology, Box 355325, Seattle, WA, 98195, USA
2 - University of Washington, Department of Biology, CAMPUS BOX 355325, SEATTLE, WA, 98195-5325, USA

Keywords:
Phylogenetics
Lamiales
Pentatricopeptide repeat gene family
primers.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PSY047
Abstract ID:462
Candidate for Awards:None


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