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Abstract Detail

Polyploidy, microevolution and species level systematics

Suda, Jan [1], Kalůsková, Jana [1], Kolář, Filip [1], Lučanová, Magdalena [1], Urfus, Tomáš [1], Vít, Petr [1], Trávníček, Pavel [1].

Polyploidy, microevolution and species level systematics: advances made by DNA flow cytometry.

Flow cytometry (FCM) is a high-throughput technology that simultaneously measures and analyses multiple parameters of individual particles such as cells, nuclei or chromosomes. Over the last decade, applications of FCM in plant biosystematics and population and evolutionary biology have expanded dramatically both in frequency and scope, primarily addressing questions of phenotypic manifestation, spatial distribution and evolutionary significance of genome duplication (polyploidy) and chromosomal variation (aneuploidy). The unsurpassed speed and reliability of estimating differences in nuclear DNA content by FCM paves the way for large-scale surveys at the landscape, population, individual and tissue levels. Representative sampling has made it possible to gain novel insights into the extent of intra- and inter-population ploidy variation, niche differentiation, and ecological preferences of particular cytotypes. The technique is also ideally suited for the detection and quantification of rare evolutionary events. Another attractive feature of FCM is the possibility to reformulate former taxonomic concepts and propose robust classifications based on a detailed understanding of population structure and phenotypic variation of polyploid groups under investigation. Discrimination among homoploid taxa and their hybrids, based on differences in genome size, is another unique aspect of FCM. In combination with molecular and phenotypic approaches, FCM promises qualitative advances in our understanding of the role of genome duplication and chromosomal variation in the evolution of vascular plants. Particular research avenues will be documented by case studies from various geographic regions and strengths and limitations of the technique will be summarized.

Broader Impacts:

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1 - Charles University In Prague, Faculty of Science, Dept. of Botany, Benátská 2, Prague, N/A, 128 01, Czech Republic

flow cytometry
genome size

Presentation Type: Symposium or Colloquium Presentation
Session: C9
Location: Belle-Chasse/Riverside Hilton
Date: Wednesday, July 31st, 2013
Time: 1:30 PM
Number: C9001
Abstract ID:266
Candidate for Awards:None

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