Abstract Detail

Genetics Section

Ravirajan, Mangaiyarkarasi [1], Murugan, Girija [1], Seenuvasan, Gnanamurthy [1].

Mutagenic effectiveness and efficiency of gamma rays and Ethyl methane sulphonate in Catharanthus roseus(L.)G.Don. * R. MANGAIYARKARASI¹ *M. GIRIJA and S.GNANAMURTHY.

Mutagenic effectiveness and efficiency of gamma rays and Ethyl Methane Sulphonate were studied in the genotype of Catharanthus roseus. The plant Catharanthus roseus is a perennial and self-pollinated plant, is of immense medicinal value due to antipotential activities of its leaf alkaloids. A mutation breeding programmed was carried out to design a physiologically and chemically efficient plant type with increased production of secondary metabolites. The mutagenic treatments seeds were tested for lethal dose 50 percent for all mutagens, separately and the dose at which 50 percent of the seed germination was considered as LD₅₀ values. Gamma rays and EMS are produced a high frequency as well as a wide spectrum of mutation. The frequency of mutation was more in EMS than gamma rays. The mutagenic effectiveness and efficiency was calculated based on biological damage. In M₁ generation based on seed lethality (L) and seedling injury (I) and M₂ generation was carefully screened for various chlorophyll and viable mutations. Mutagenic effectiveness and efficiency increased with the decreased in dose or concentration. In the present study EMS was provide to be more effective and efficient in causing mutations as compared to gamma rays treatments. Key words: Mutagens, Effectiveness, Efficiency, Gamma rays, EMS, Catharanthus roseus.

Broader Impacts:

1 - Annamalai University, Botany, Chidambaram, Chidambaram, (None), 608001, India

Keywords:
Mutagens
Effectiveness
Efficiency
Gamma rays
EMS
Catharanthus roseus.

Presentation Type: Oral Paper:Papers for Sections
Session: 6
Location: Marlborough B/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 11:15 AM
Number: 6010
Abstract ID:255
Candidate for Awards:Margaret Menzel Award