| Abstract Detail
Recent Topics Posters Van Kley , Alexandra Martynova [1], Van Kley, James [1], Nalian, Armen [1], Kurmaeva, Elvira [1], Patnaik, Sikta [1]. Relationships Between Arbuscular Mycorhhizal Fungi, Host Plant, and Habitat in Eastern Texas, USA Forests. We used molecular techniques to describe communities of arbuscular mycorrhizal fungi (AMF) from three different host plants for a range of eastern Texas USA forest habitats. We used denaturing gradient gel electrophoresis (DGGE) to separate AMF PCR products of 18S rDNA amplified from DNA extracted from the root samples. A subset of the samples were also subjected to 454 pyrosequencing. Both the DDGE and pyrosequencing results indicated that AMF communities varied with habitat and with factors including topographic position, soil texture and several soil nutrients. Most of the AMF taxa we identified from sequences obtained from pyrosequencing using the GenBank sequence database and BLAST occurred on more than one host plant. However, certain ones showed preferences and the overall AMF community differed by host. Broader Impacts:
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1 - Stephen F Austin State University, 1936 North Street, Nacogdoches, TX, 75962-3003, United States
Keywords:
arbuscular mycorrhizal fungi (AMF)
PCR
18S rDNA
pyrosequencing
DDGE
host plants.
Presentation Type: Recent Topics Poster
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PRT006
Abstract ID:1292
Candidate for Awards:None |
